❞ كتاب Mammalian Artificial Chromosomes_ Methods and Protocols ❝ ⏤ Vittorio Sgaramella Sandro Eridani

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From Natural to Artificial Chromosomes
An Overview
Vittorio Sgaramella and Sandro Eridani
1. Introduction
The rationale for building artificial chromosomes (ACs) has been
critically reviewed by various authors at different stages of this line
of investigation, initiated in the early 1980s.
Two major goals have been generally stressed: first, the possibility of a better understanding of the structure and functions of natural chromosomes; second, the challenges presented by their use as
large-capacity gene vectors for DNA cloning, in view of genetic
improvement (in animals of commercial relevance) and hopefully
of disease correction (in humans): for a review, see Willard (1). The
main features for an efficient mammalian AC (MAC) are (1) a vectorial capacity up to a few megabases; (2) a manageable size for
their in vitro manipulation; (3) a correct intracellular location and
copy number; (4) no untoward effect on the host cell; and (5) the
ability to express the transgene (or transchromosome) in a physiological way

Biology
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