❞ كتاب Natural Killer Cell Protocols_ Cellular and Molecular Methods-Humana Press ❝ ⏤ Kerry S. Campbell Marco Colonna

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Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.

Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
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Ecology or environmental science

نبذه عن الكتاب:

1. Introduction
Our understanding of the phenotypical and functional heterogeneity of
human natural killer (NK) cells has greatly advanced over the past few years.
This advancement has been greatly helped by the development of culture conditions for clonal proliferation of NK cells. Analysis of human NK cell clones
has led to the original observation that different NK cell clones recognize distinct
major histocompatibility complex (MHC) class I specificities. This has prompted
the production of monoclonal antibodies directed to NK cell surface antigens
clonally distributed, and, ultimately, the biochemical and molecular definition of
the NK cell surface glycoproteins functioning as MHC class I receptors.
Here we describe a limiting dilution culture protocol that allows establishment of human NK cell clones from peripheral blood leukocytes with high
efficiency.
2. Materials
1. RPMI 1640 (Gibco, cat. no. 31870-025).
2. RPMI + HEPES (25 mM): (Gibco, cat. no. 42401-018).
3. Lymphocyte separation medium (LSM): (ICN Biomedicals, cat. no. 50494/36427).
4. Human recombinant interleukin (IL)-2 Liquemin (25000 UI/5 mL, Roche).
5. RPMI 8866 cells (available from Dr. Bice Perussia, Jefferson Medical College, Kimmel
Cancer Institute, BLSB 750, 233 S 10th Street, Philadelphia, PA 19107, USA).
6. Antibodies: Anti-human CD56 (Pharmingen, cat. no. 31661A, mouse IgG1), antihuman CD3 (OKT3, mouse IgG2a, ATCC, cat. no. CRL-8001), anti-mouse IgG1-
phycoerythrin (PE; SBA, cat. no. 1070-09), anti-mouse IgG2a-fluorescein
isothiocyanate (FITC; SBA, cat. no. 1080-02).
7. Phosphate-buffered saline (PBS).
8. PBS supplemented with 1% fetal calf serum (FCS).

Biology
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Research on biology for the first grade of secondary school
Human biology